|
KMID : 0358420150580030232
|
|
Korean Journal of Obstetrics and Gynecology
2015 Volume.58 No. 3 p.232 ~ p.238
|
|
|
Relationship between phospholipase C zeta immunoreactivity and DNA fragmentation and oxidation in human sperm
|
|
Park Ju-Hee
Kim Seul-Ki
Kim Ja-Yeon
Kim Ji-Hee
Chang Jae-Hoon
Lee Byung-Chul
Kim Seok-Hyun
|
|
|
Abstract
|
|
|
Objective: The study aimed to evaluate the feasibility and reproducibility of measuring phospholipase C zeta (PLCζ) using immunostaining in human sperm and to investigate the relationship between PLCζ immunoreactivity and DNA fragmentation and oxidation in human sperm.
Methods: Semen samples were obtained from participants (n=44) and processed by the conventional swim-up method. Sperm concentration, motility, normal form by strict morphology, DNA fragmentation index assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling method and immunofluorescent expression for 8-hydroxy-2’-deoxyguanosine (8-OHdG) and PLCζ were assessed.
Results: When duplicate PLCζ tests were performed on two sperm samples from each of the 44 participants, similar results were obtained (74.1±9.4% vs. 75.4±9.7%). Two measurements of PLCζ were found to be highly correlated with each other (r=0.759, P<0.001). Immunoreactivity of PLCζ was not associated with donor’s age, sperm concentration, motility, and the percentage of normal form as well as DNA fragmentation index. However, immunoreactivity of PLCζ showed a significant negative relationship with 8-OHdG immunoreactivity (r=-0.404, P=0.009).
Conclusion: Measurement of PLCζ by immunostaining is feasible and reproducible. Lower expression of PLCζ in human sperm may be associated with higher sperm DNA oxidation status.
|
|
|
KEYWORD
|
|
|
DNA oxidation, 8-Hydroxy-2’-deoxyguanosine, Phospholipase C zeta, Spermatozoa
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|